Plasmid DNA preps from glycerol stocks in advance of sequencing
PCR Products Sequencing
Bio Basic Advantages
Repeat Sequencing – Other service providers will usually only provide a single run.
Courtesy Gel Analysis – Our team will perform a courtesy gel analysis.
Positive controls – Included to ensure proper sequencing chemistry and procedures are carried out.
No strict guidelines for sample layout – We accept samples in many formats and arrangements. We will not refuse an order due to orientation and will gladly work with you to sort things out. Of course we would appreciate it if you would arrange your samples in a certain order (column by column or row by row).
No penalties –We understand that not everything works out, samples may be compromised during transit. We do not charge for reactions we do not perform.
Very competitive pricing.
Free shipping for more than 100 reactions.
Samples are accepted as bacterial colonies, glycerol stocks, pelleted cultures and crude PCR products.
Multiple of 3730XL sequencers stand by
Sequence up to 800bp per reaction, more than 90% successful rate
Special prices on large projects and contracts
24hr to 48 hour turnaround
Incoming sample QC
Fluorescent dye-terminator sequencing
>650bp Q20 read lengths typical
Universal primers provided at no additional charge (complete list)
Template preparation and primer synthesis available
Sequencing report format (1) Original ABI format of ~700bp sequencing chromatogram (2) Summary of Report (3) All results are sent electronically
View results (1) Download Chromas from www.biobasic.com (2) Double click Chromas and open ABI file from Chromas (3) Export text file from Chromas (4) Sequences between 60bp to 500bp are most reliable region. To obtain reliable reads, it is highly recommended to perform double stranded sequencing using reverse primers as a good laboratory practice.
No report (1) Samples fail to pass incoming QC (2) Sample with no readable signal
Charges on sequences with structures Standard charge applies even if sequences are not “clear” due to known structures including Poly N?GC?inverted repeats direct repeats etc.
Charges on mixed peaks Most “mixed” peaks are results from “mixed” templates (see below Q&A). Standard charge applies.
Sample Storage All samples are kept at Bio Basic Inc. for 1 month. During this period, customer may request additional sequencing service. After 1 month, the samples are automatically destroyed without further notification.