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   Oligo Synthesis


To request a quotation or for placing orders, please download our Excel order form (preferred) and email it to synthesis@biobasic.com. Our representative will get back to you within 24 hours (during a business day; EST).

For a downloadable PDF overview of our Oligo Synthesis service, please click here.

Purification Types:


DESALTED
>55% Purity*
  • Salts Removed.
  • Failure sequences are NOT removed.

HAP
>75% Purity*
  • Bio Basic’s proprietary method of High Affinity Purification (HAP).
  • Oligos significantly purer than the Desalted method.
  • Salts removed.
  • Some failure sequences removed.

HAP+MS
>75% Purity*
  • Bio Basic’s proprietary method of High Affinity Purification (HAP).
  • Oligos significantly purer than the Desalted method.
  • Salts removed.
  • Some failure sequences removed.
  • MS Data provided.

PAGE
>85% Purity*
  • Polyacrylamide gel electrophoresis (PAGE) based purification.
  • Recommended for longer oligos.
  • Salts removed.
  • Majority of failure sequences removed.
  • MS data provided.

HPLC
>85% Purity*
  • High Performance Liquid Chromatography (HPLC) based purification.
  • Recommended for shorter oligos.
  • Salts removed.
  • Majority of failure sequences removed.
  • MS data provided.

HPLC-CE
>85% Purity*
  • HPLC with Capillary Electrophoresis (CE).
  • Guaranteed minimum purity of 85% ensured.
  • Recommended for shorter oligos.
  • Salts removed.
  • Majority of failure sequences removed.
  • MS data provided.

*: Based on a 30nt oligo. Longer oligos refer to oligos greater than 50nt and shorter oligos refer to oligos less than 50nt in length.

Features:

High purity oligos at highly competitive rates. Available in Guaranteed OD packages. QC and Quality Guaranteed.
Dedicated technical support staff. Wide range of modifications available. Price match and counteroffer Guarantee.

High Affinity Purification (HAP) is a patented, novel purification method for custom oligos. DMT-ON-Oligo in the crude oligo mixture is first selectively absorbed on a high affinity resin in HAP column while incomplete oligos pass through. Final oligo is produced by removing the protection group of DMT under mild acidic conditions. HAP method provides two major advantages, high purity superior to De-Salted method (>98%), and low cost comparable to PAGE or HPLC methods. Oligos produced by the HAP method has such high purity that they can be directly used for any downstream experiments such as PCR, DNA Sequencing, Gene Synthesis, and Mutagenesis. At present, the most economic method to produce oligos is the De-Salted method, which however, yields products poor in purity. For example, the purity of 20-mer, 40-mer and 60-mer is approximately 68%, 45% and 30% respectively. This is calculated based on the 4 steps in DNA synthesis: De-DMT, Coupling, Oxidation and Capping. The average yield of each cycle is about 98%. The purity of 20-mer is therefore (0.98)20-1 = 68%; of 40-mer = (0.98)40-1 = 45%; and of 60-mer = (0.98)60-1 = 30%. Most laboratories use De-Salted oligos despite of their inferior purity because higher quality oligos produced by alternative methods such as PAGE, HPLC, and OPC are too costly. HAP presents the perfect alternative for high purity oligos at lowest prices. In fact, price is even lower than those of De-Salted method in some cases.

More Information

  • When ordering oligos: Remember that the scale of synthesis (25 nmol, 100 nmol, etc.), refers to the starting material used to synthesized the oligo, NOT the FINAL yield. Oligo length, sequence, GC content and coupling efficiencies can vary the final nmole yield post-synthesis. Different purification methods will also alter the final product yield. Bio Basic guarantees the final yield of each oligo nucleotide as a FIXED final yield in  OD units.
  • For more information, please contact our oligo department via email at synthesis@biobasic.com or call 1 (800) 313-7224 ext.329.

How to Order

Order Notes

Please note for EACH oligo, we will need the following (as indicated in the order forms):

  • 1) Unique oligo name.
  • 2) Sequence (5' to 3').
  • 3) Final yield (in OD).
  • 4) Indicate purification type (Desalt, HAP, PAGE, HPLC or HPLC-CE).
  • 5) Modifications (if any) in their respective location.