For a downloadable PDF overview of our Protein Purification service, please click here.
Protein Expression and Purification service
Guaranteed 15 mg purified soluble protein for only $2,880!
General Summary of our Process
We have three expression systems:
1. E.Coli Expression System
2. Baculovirus Expression System
3. Mammalian Expression System
1. For E.Coli expression projects, we have a standard pricing and they come in guaranteed packages of 1mg, 5mg and 15mg of purified protein.
2. or 3. In the case of the Baculovirus or Mammalian expression projects, we start with a pilot study (please inquire about pricing) in order to analyze whether the expression would be high or low. Then, based on the results of the pilot study, we determine pricing for the subsequent full-length project.
After the pilot study, you can choose whether to continue with the project, should you be satisfied with the price and pilot study results.
Projects can either be initiated from gene synthesis, or you can simply provide us with the constructs that you would like to express.
At the end of the project, we deliver all of the products we guarantee (amount of protein or study results).
For more information on pricing and order placement, see below, or contact us directly by email or phone at firstname.lastname@example.org; (905) 474 4493.
Bio Basic Inc. in gene, peptide synthesis, is pleased to launch our protein expression and purification service. Bio Basic Inc. has the necessary know-how to deliver quality results to our valued customers. We have the capacity to take your project directly from gene synthesis to protein expression, refolding and purification. At Bio Basic Inc., we are proud of our ability to optimize protein expression, maximize product yield, and ensure purity to serve every aspect of your research needs.
From Gene to Protein
Starting from de novo gene synthesis, cDNA or from almost anywhere a client asks us to begin; our scientists can deliver high quality purified protein in a timely manner.
About Protein Purification
Recombinant proteins are used throughout the whole spectrum of biomedical research. Once you have large quantity of purified protein, you could conduct many downstream experiments, such as enzyme kinetics, functional and structural studies.
There are two main systems for the expression of recombinant protein - prokaryotic and eukaryotic.
Prokaryotic recombinant protein expression systems (E.Coli) have several advantages, including ease of culture, rapid cell growth and relatively simple purification procedures. However, most post-translational modifications are not added by bacteria, and most proteins become insoluble in inclusion bodies and are very difficult to recover as functional proteins.
Eukaryotic systems for the expression of protein include yeast, baculovirus cells, and mammalian cells. Advantages of these systems include high levels of expression, no inclusion bodies, and intact post-translational modifications.
Things to Consider
Sequence Optimization: In highly expressed genes, a narrow set of codons is used and these codons correspond to the more abundant tRNA species. This minimizes the risk of tRNA depletion during translation. In fact, the codons in a gene may be true bottlenecks, especially in cases where foreign genes are expressed in a host in which the usage of codons in highly expressed genes does not resemble the usage of codons in the species from which the foreign gene originates. In such cases, it has been shown that substitution of rare codons in the introduced gene may increase the yield dramatically. In addition, replacement of rare codons might decrease the chance of misincorporation and protect the protein from premature turnover (1).
Expression System: In order to decide which host is most suitable the amount and the degree of purity of the product as well as its biological integrity and potential toxicity should be considered. For example, bacterial expression systems are not suitable if post-translational modification is required to produce a fully functional recombinant product (2).
Expression Vector: Once the host has been selected, many different vectors are available for consideration. However, as for the selection of a suitable host system, the final choice of vector should take into consideration the specific requirements of the application and will, of course, be influenced by the behaviour of the target protein.
Tag: As for the selection of host and vectors, the decision to use which tag must be made according to the needs of the specific application. The two most commonly used tags are glutathione S-transferase (GST tag) and 6 x histidine residues ((His)6 tag).
Upon receiving of sequence information, our scientists would analyze the sequence to determine characteristics like molecular weight, pI, solubility, susceptibility to oxidation and hydrophobicity. Every protein is different, there is no standard way for protein expression and purification, and the protocols and strategies must be worked out for each individual protein and with its intended use in mind. Scientists at Bio Basic Inc. use their accumulated extensive working knowledge of purifying recombinant protein to best tailor our service to serve your research needs. At Bio Basic Inc., we offer all four expression systems* to give you the maximum flexibility.
Protein Expression Systems at Bio Basic Inc.
1. E.Coli Expression System Escherichia coli (E. coli) is one of the most widely used hosts for the production of heterologous proteins. Its genetics are far better characterized than those of any other microorganism.
2. Baculovirus Expression System The Baculovirus insect cell expression system is widely used for the production of precisely matured, folded and processed recombinant proteins. In contrast to E. coli, insect cells are able to incorporate post-translational modifications. This unique tool usually yields high amounts of the target protein, making it highly cost effective in comparison to other eukaryotic expression systems.
3. Mammalian Expression System Mammalian cell lines (CHO, HEK 293 etc) are widely used for the production of recombinant glycoproteins (vaccines, enzymes) as well as hormones and immunobiologicals (antibodies, interleukins, lymphokines), as they have the advantage of full posttranslational modifications of the expressed protein.
* Yeast expression system is available upon request.
Price and Services Summary
1. E.Coli Expression System
Guaranteed 15 mg purified soluble protein (Cat# PUR-15MG-ECOLI) Subcloning Expression optimization and verification Expression and purification QC by SDS-PAGE, Western blot* Tag removal, endotoxin removal service available
Guaranteed 15 mg purified soluble protein from E.Coli expression system QC data
Guaranteed 5 mg purified soluble protein (Cat# PUR-5MG-ECOLI) Subcloning Expression optimization and verification Expression and purification QC by SDS-PAGE, Western blot* Tag removal, endotoxin removal service available
Guaranteed 5 mg purified soluble protein from E.Coli expression system
Traditional Service (Cat# PUR-TRAD) Subcloning Expression optimization and verification Expression and purification QC by SDS-PAGE, Western blot* Tag removal, endotoxin removal service available
Purified protein from E.Coli expression system
The guaranteed Packages (PUR-15MG-ECOLI & PUR-5MG-ECOLI) have the following features:
Customer provides the plasmid or the expression strain
Size of the target protein is usually <100KDa
In some rare cases, in order to facilitate expression, Bio Basic Inc. may try different tags and final protein product may contain such tag
The final purified protein is tag-containing
Bio Basic Inc. does not guarantee the endotoxin level of purified protein
Bio Basic Inc. guarantees the protein to be soluble, but not the bioactivity or functionality. If target protein expresses as inclusion body, refolding will be done to make it soluble.
If Bio Basic Inc. failed to deliver the guaranteed amount due to the nature of the protein or other unpredictable circumstances, only a minimum set up fee will apply
An Exciting New Launch 5 mg Intact-Native Protein Expression for only $5,200!
5 mg of purified protein in its native form (No Tag, 90- 95% purity, endotoxin
Intact Native Protein Expression Package (Cat#INT-5MG-ECOLI) Subcloning Expression optimization and verification Expression and purification Ultrasonication, Refolding, Purification, Tag Removal QC by SDS-PAGE, Western blot*
5 mg of purified protein in its native form (No Tag, 90- 95% purity, endotoxin <1eu/ug)
* Certain restrictions apply. Please inquire for details.
2. Other E. Coli Expression System Services
Functional preservation and longer shelf life
Scale-up of fermentation and purification process up to 300 liters
cGMP manufacturing services
Fully equipped cGMP facility
3. Baculovirus Expression System (Cat# PUR-BACUV)
Subcloning Generation of recombinant bacmid DNA Generation of high-titer viral stock Infection of insect cells with high-titer virus stocks QC by SDS-PAGE, Western blot* Tag removal service available
High-titer viral stock Purified soluble protein QC data
4. Mammalian Expression System (Cat# PUR-MAML)
Transient expression and purification Stable cell line establishment QC by SDS-PAGE, Western blot* Tag removal service available
* FREE Western QC data is available for tagged proteins that can be detected by the anti-His antibody. For other tagged proteins, additional charges may apply.
Featured Catalogue Products
In addition to the outlined custom protein purification services, Bio Basic Inc. possesses an extensive list of ready made proteins. This following table includes a sampling of the catalogue protein library (We have a lot more!). If your protein of interest is not on the list, please send an email to email@example.com to inquire.
Other Specialty Products
Recombinant Human IL-1α
Recombinant Human GM-CSF
Recombinant Human SDF-1α/CXCL12
Recombinant Human IFN-α1b
Recombinant Human TNF-α
Recombinant Human IL-2
Recombinant Human EPO
Recombinant Human Lymphotactin/XCL1
Recombinant Human IFN-α2b
Recombinant Bovine Enterokinase
Recombinant Murine IL-3
Recombinant Human IGF-1
Recombinant Human RANTES/CCL5
Recombinant Human IFN-γ
Recombinant Human IL-12
Recombinant Human TPO
Recombinant Human MIP-1α/CCL3
Recombinant Human IL-29/IFN-λ1
Recombinant Cys-Protein G
Recombinant Human IL-33
Recombinant Human EGF
Recombinant Human GRO-α/CXCL1
Recombinant Rat IFN-γ
Recombinant Protein A/G
Contact: To inquire or request a quote, please complete the Protein Order Form and send an email to firstname.lastname@example.org.